Bioaccumulation of Cadmium, Copper, and Lead in Edible Tissues of Clarias gariepinus and Oreochromis niloticus from Anwai River, Nigeria

Authors

  • Festus Ovwighose Onadje Department of Animal and Environmental Biology, Dennis Osadebay University, Asaba
  • Augustine Ikechukwu Chukwurah Department of Environmental Management and Toxicology, Dennis Osadebay University, Asaba
  • Donald Uzowulu Olannye Department of Environmental Management and Toxicology, Dennis Osadebay University, Asaba
  • Kesiena Efe-Eyefia Department of Biochemistry and Molecular Biology, Dennis Osadebay University, Asaba
  • Uzome James Oghene Department of Environmental Management and Toxicology, Dennis Osadebay University, Asaba

DOI:

https://doi.org/10.63561/jacsr.v2i4.1020

Keywords:

Water Pollution, Heavy Metal, Aquatic habitat, Anwai River, Oreochromis niloticus, Clarias gariepinus

Abstract

Environmental pollution is a continuous threat to the quality of aquatic habitats for plants and animals. This study aims to evaluate the concentrations of selected heavy metals (Cd, Cu, and Pb) in the flesh, liver, gills, and stomach of Clarias gariepinus and Oreochromis niloticus collected from the Anwai River, South-South Nigeria. Ten fish, each of both species measuring 17.25±1.70cm and 19.42±2.36cm respectively, were obtained and analysed using the Atomic Absorption Spectrophotometer. The collected data were subjected to one-way analysis of variance (ANOVA) at the 5% significance level. Results revealed that Lead concentrations were highest in all organs of the fish species, while the concentration of Cadmium was least. Heavy metals were lower in Oreochromis niloticus than in Clarias gariepinus. The concentrations of the metals investigated in both cases ranged from 0.09 mg/kg to 4.56 mg/kg and were far above regulatory permissible limits set by international standards, such as those of the World Health Organisation (WHO). The results of this study may indicate heavy metal pollution in the Anwai River and highlight the possible risk of eating fish from the river. Therefore, continuous evaluation of the Anwai River’s pollution levels is necessary to reduce it through effective campaigns and sensitisation.

References

Adegbola, I. P., Aborisade, B. A., & Adetutu, A. (2021). Health risk assessment and heavy metal accumulation in fish species (Clarias gariepinus and Sarotherodon melanotheron) from industrially polluted Ogun and Eleyele Rivers, Nigeria. Toxicology Reports, 8, 1445–1460.

Ayanda, I. O., Ekhator, U. I., & Bello, O. A. (2019). Determination of selected heavy metals and proximate composition in some fish species from Ogun River, southwestern Nigeria. Heliyon, 5(10), e02512. https://doi.org/10.1016/j.heliyon.2019.e02512

Bautista, C. J., Arango, N., Plata, C., Mitre-Aguilar, I. B., Trujillo, J., & Ramírez, V. (2024). Mechanism of cadmium-induced nephrotoxicity. Toxicology, 502, 153726. https://doi.org/10.1016/j.tox.2024.153726

Bawuro, A. A., Voegborlo, R. B., & Adimado, A. A. (2018). Bioaccumulation of heavy metals in some tissues of fish in Lake Geriyo, Adamawa State, Nigeria. Journal of Environmental and Public Health, 2018, 1854892. https://doi.org/10.1155/2018/1854892

Chukwurah A. I., Uruh K., Olannye D. U., Emetulu V. C. and Enamuotor O. (2023). Effects of Water Pollution on Aquatic Life: A Case Study on River Ethiope. Journal of Scientometrics and Innovations. 1 (1): 210 – 220.

Edojarievwen, U. T., Chukwurah, A. I., Emetulu, V. C., Kesiena, U., & Onoriasakpobare, F. O. (2025). Assessment of water quality suitability for sustainable aquaculture using water pollution index: A case study of Anwai River, Southern Nigeria. Current Research in Plant Science, 1(1), 18–23. https://doi.org/10.58613/crps113

Egun, N. K., Okotie, U. R., & Oboh, I. P. (2023). Distribution and toxicological risk assessment of metals in commercial fish species from a lentic ecosystem in Nigeria. Journal of Trace Elements and Minerals, 6, 100100. https://doi.org/10.1016/j.jtemin.2023.100100

Ejike, L. O., & Liman, M. G. (2017). Determination of heavy metals in selected fish species from Kwalkwalawa River, Sokoto State, Nigeria. IOSR Journal of Applied Chemistry, 10(1), 38–42. https://doi.org/10.9790/5736-1001023842

FAO. (1983). Manual of methods in aquatic environmental research. Food and Agriculture Organization of the United Nations.

Garai, P., Banerjee, P., Mondal, P., & Saha, N. C. (2021). Effect of heavy metals on fishes: Toxicity and bioaccumulation. Journal of Clinical Toxicology, S(18).

Hamada, N. H., Ali, R. A., El-Tabakh, M. A., & Bream, A. S. (2024). Environmental monitoring: Tobara fish as bioindicators of heavy metal pollution in a coastal ecosystem. Regional Studies in Marine Science, 69, 103335. https://doi.org/10.1016/j.rsma.2023.103335

Hussein, M. A., Morsy, N. S., Mahmoud, A. F., Darwish, W. S., Elabbasy, M. T., Zigo, F., Farkašová, Z., & Rehan, I. F. (2023). Risk assessment of toxic residues among freshwater and marine fish species. Frontiers in Veterinary Science, 10, 1185395. https://doi.org/10.3389/fvets.2023.1185395

Iloba, K. I., & Adamu, K. M. (2020). Ecological responses of macroinvertebrates to human impacts of a rural–urban flowing river in Delta State, Nigeria. Journal of Biodiversity and Environmental Sciences, 16(4), 10–18.

Inderhaug, T. (2020). Stockfish production, cultural and culinary values. Food Ethics, 5(1), 6. https://doi.org/10.1007/s41055-020-00069-5

Izuchukwu, I. U., Okeke, D. O., & Okpashi, V. E. (2017). Determination of heavy metals in fish tissues, water and sediment from River Niger, Nigeria. Journal of Environmental & Analytical Toxicology, 7(5), 1–3.

Lee, J. W., Park, J. S., & Choi, Y. J. (2024). Cadmium bioaccumulation and organ distribution in freshwater fish: A mechanistic review. Toxics, 12(1), 34. https://doi.org/10.3390/toxics12010034

Lee, S., Mamun, M., Atique, U., & An, K. (2023). Fish tissue contamination with organic pollutants and heavy metals: Link between land use and ecological health. Water, 15(10), 1845. https://doi.org/10.3390/w15101845

Li, J., Sun, C., Zheng, L., Jiang, F., Wang, S., Zhuang, Z., & Wang, X. (2017). Determination of trace metals in tropical marine fishes from the Spratly Islands. Marine Pollution Bulletin, 122(1–2), 464–469. https://doi.org/10.1016/j.marpolbul.2017.06.017

Mahamood, M., Khan, F. R., Zahir, F., Javed, M., & Alhewairini, S. S. (2023). Fish and aquatic plants as bioindicators of heavy metal pollution. Scientific Reports, 13, 28313. https://doi.org/10.1038/s41598-023-28313-9

Mebane, C. A., Schmidt, T. S., & DeForest, D. K. (2023). Copper bioavailability and toxicity in freshwater fish. Environmental Toxicology and Chemistry, 42(4), 1001–1015. https://doi.org/10.1002/etc.5589

Mokarram, M., Saber, A., & Sheykhi, V. (2020). Effects of industrial effluents on river water quality. Journal of Cleaner Production, 277, 123380. https://doi.org/10.1016/j.jclepro.2020.123380

Moussa, M. A., Okoye, B. C., & Afolabi, A. A. (2022). Heavy metal accumulation and DNA damage in Oreochromis niloticus. Scientific Reports, 12, 21104. https://doi.org/10.1038/s41598-022-25417-7

Nordberg, M., & Nordberg, G. F. (2022). Metallothionein and cadmium toxicology. Biomolecules, 12(3), 360. https://doi.org/10.3390/biom12030360

Nwude, D. O., Babayemi, J. O., & Ajibode, C. P. (2020). Heavy metal levels in freshwater fish from Ogun River, Nigeria. Journal of Applied Sciences and Environmental Management, 24(8), 1433–1440.

Odesa, G. E. & Olannye, D. U. (2024). Health Risk Assessment of PAHs and Heavy Metal Levels in Periwinkles (Pachymelania fusca mutans) and Crabs (Scylla serrata) Consumed in Crude Oil-Contaminated Coastal Regions of Southern Nigeria. Toxicology Reports. 14 : 101852. https://doi.org/10.1016/j.toxrep.2024.101852

Olayinka-Olagunju, J. O., Dosumu, A. A., & Olatunji-Ojo, A. M. (2021). Bioaccumulation of heavy metals in pelagic and benthic fishes of Ogbese River, Ondo State, South-Western Nigeria. Water, Air, and Soil Pollution, 232, 44. https://doi.org/10.1007/s11270-021-04987-7

Onadje, F.O., Chukwurah, A., I.& Agbeyi, E.A. (2025). Multiple Biomarkers Variability in Lead Exposed Fresh Water Fish (Clarias gariepinus). Journal of Advances in Biology & Biotechnology 28 (4):513-20. https://doi.org/10.9734/jabb/2025/v28i42209.

Onadje, F. O., & Akalusi, K. E. (2024). Biochemical effects of lead exposure in Clarias gariepinus. FNAS Journal of Applied Biological Sciences, 2(1), 79–89.

Rahman, Z., & Singh, V. P. (2019). Impact of toxic heavy metals on the environment. Environmental Monitoring and Assessment, 191, 419. https://doi.org/10.1007/s10661-019-7528-7

Rama Jyothi, N. (2021). Heavy metal sources and their effects on human health. In Heavy metals – Their environmental impacts and mitigation. https://doi.org/10.5772/intechopen.95370

Salem, T. A. (2021). Changes in physicochemical and biological characteristics of Nile River waters. Egyptian Journal of Aquatic Research, 47, 21–27.

Segaran, T. C., Azra, M. N., Piah, R. M., Lananan, F., Téllez-Isaías, G., Gao, H., & Noordin, N. M. (2023). Catfishes: A global review. Heliyon, 9(9), e19135.

Shahjahan, M., Rahman, M. S., Islam, S. M., & Uddin, M. H. (2022). Heavy metal accumulation and health risks in freshwater fish. Environmental Science and Pollution Research, 29, 35612–35625.

Siddhnath, Sharma, S., & Singh, A. (2024). Dried fish and its role in the global economy. In Dry fish: A global perspective on nutritional security and economic sustainability (pp. 59–76). Springer Nature.

Sun, Z., Xie, X., Wang, P., Hu, Y., & Cheng, H. (2018). Heavy metal pollution from small-scale mining. Science of the Total Environment, 639, 217–227. https://doi.org/10.1016/j.scitotenv.2018.05.235

Vareda, J. P., Valente, A. J., & Durães, L. (2019). Heavy metal pollution assessment and remediation strategies. Journal of Environmental Management, 246, 101–118. https://doi.org/10.1016/j.jenvman.2019.05.126

Wanjari, U. R., & Gopalakrishnan, A. V. (2024). Cadmium as a male reproductive toxicant. Environmental Science and Pollution Research, 31(12), 18340–18361. https://doi.org/10.1007/s11356-024-32210-7

WHO. (2003). Codex alimentarius: Maximum levels of heavy metals in fish. World Health Organization.

WHO. (2023). Evaluation of certain contaminants in food. World Health Organization.

Winiarska-Mieczan, A., Florek, M., Kwiecień, M., Kwiatkowska, K., & Krusiński, R. (2017). Cadmium and lead content in fishery products. Biological Trace Element Research, 182(2), 373–380. https://doi.org/10.1007/s12011-017-1104-1

Yilmaz, F., Ozdemir, N., Demirak, A., & Tuna, A. L. (2007). Heavy metal levels in two fish species. Food Chemistry, 100, 830–835.

Zhuzzhassarova, G., Sarsembayeva, N., & Duisenbekova, B. (2024). Fish and seafood safety: Human exposure to toxic metals. Environmental Advances, 16, 100328. https://doi.org/10.1016/j.envadv.2024.100328

Published

2025-12-30

How to Cite

Onadje, F. O., Chukwurah, A. I., Olannye, D. U., Efe-Eyefia, K., & Oghene, U. J. (2025). Bioaccumulation of Cadmium, Copper, and Lead in Edible Tissues of Clarias gariepinus and Oreochromis niloticus from Anwai River, Nigeria. Faculty of Natural and Applied Sciences Journal of Applied Chemical Science Research, 2(4), 52–59. https://doi.org/10.63561/jacsr.v2i4.1020

Similar Articles

<< < 1 2 3 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)