Growth Response of Two Cowpea Varieties to the Allelopathic Effects of Azadirachta indica (A. Juss) Bark Extract

Authors

  • Patience Onize Etuwoma etuwoma.patience@dou.edu.ng
  • Ichehoke Austine Omakor Department of Plant Science and Biotechnology, Dennis Osadebay University, Asaba

DOI:

https://doi.org/10.63561/jabs.v2i4.1009

Keywords:

Azadirachta indica, Vigna unguiculata, Allelopathy, Bark extracts, Cowpea

Abstract

The aim of this study was to evaluate the growth response of two varieties of Cowpea – Vigna unguiculata (SAMPEA 7 and IT97K-499-35) to the bark extract of Azadirachta indica. The crop plant was exposed to varying concentrations of 100, 75, 50, and 25 g/L of the aqueous stem bark extract using a Complete Randomised Block Design. There were three replications per treatment, of which three were randomly selected for further analysis. The study lasted 10 days. Data was collected on Germination percentage, root length, shoot length and fresh weight. Data were analysed using ANOVA at the 5% significance level, and DMRT was used to rank the means. The results revealed the susceptibility of the two cowpea varieties to the stem bark extract of A. indica, in a concentration-dependent manner, with respect to germination percentage, root and shoot length, and seedling fresh weight. The overall germination and growth rate of seedlings were reduced with the 100 g/L neem stem bark extract treatment, followed by 75 g/L, then 50 g/L; the least was 25 g/L. Variety IT97K-499-35 showed more susceptibility to the treatments. This suggests that A. indica exhibits an allelopathic effect on the examined types and can therefore serve as a biological means of weed control, as natural substances are regarded as more environmentally benign than most synthetic herbicides.

References

contaminated with spent engine oil. Journal of Environmental and Chemical Ecotoxicology, 2(7), 103–109.

Awosanya, A. O. (2024). Allelopathic effect of neem (Azadirachta indica) extracts on the germination and viability of cowpea seedlings. Journal of Science and Information Technology (JOSIT), 18(2), 193–201.

Belz, R. G., Hurle, K., & Duke, S. O. (2005). Dose–response—a challenge for allelopathy? Nonlinearity in Biology, Toxicology, and Medicine, 3(2), 173–211. https://doi.org/10.2201/nonlin.003.02.002

Ferguson, J. J., Rathinasabapathi, B., & Chase, C. A. (2013). Allelopathy: How plants suppress other plants (Publication No. HS944/HS186). UF/IFAS Extension, University of Florida.

Godwin, R. H., & Avers, C. J. (1950). The effect of coumarin derivatives on the growth of Avena roots. American Journal of Botany, 37, 224–227.

Gross, D., & Parthier, B. (1994). Novel natural substances acting in plant growth regulation. Journal of Plant Growth Regulation, 13(2), 93–114. https://doi.org/10.1007/BF00210953

Jagtap, M. B., Tayade, S. K., & Athawale, N. K. (2016). Allelopathic effects of aqueous neem (Azadirachta indica A. Juss.) leaf extract on seed germination in some crop plants. International Journal of Scientific Research, 5(2), 425–426.

Javed, K. (2020). Allelopathy: A brief review. Journal of Novel Applied Sciences, 9(1), 1–12.

Kajidu, Y. A., Ibrahim, U., & Bello, S. (2023). Allelopathic potential of neem (Azadirachta indica) on the growth and development of selected crops. Journal of Plant Science Research, 15(1), 45–54.

Kato-Noguchi, H. (2024). Isolation and identification of allelochemicals and their activities and functions. Journal of Pesticide Science, 49(1), 1–14. https://doi.org/10.1584/jpestics.D23-052

Krishnaveni, A., Kaviya, P., Vijayalakshmi, V., Vennila, S. M., Ananthi, K., Malathi, G., Sivakumar, C., Jegathambal, R., & Aneesa Rani, S. M. (2025). Study on the impact of neem (Azadirachta indica) leaf extract on the growth of field crops. International Journal of Advanced Biochemistry Research, 9(1), 31–38. https://doi.org/10.33545/26174693.2025.v9.i1a.3408

Li, J., Chen, L., Chen, Q., Miao, Y., Peng, Z., Huang, B., Guo, L., Liu, D., & Du, H. (2021). Allelopathic effect of Artemisia argyi on the germination and growth of various weeds. Scientific Reports, 11(1), Article 83752. https://doi.org/10.1038/s41598-021-83752-6

Liu, T., Li, T., & Zhang, L. (2021). Exogenous salicylic acid alleviates pesticide-induced oxidative stress in cucumber plants (Cucumis sativus L.). Ecotoxicology and Environmental Safety, 208, Article 111654. https://doi.org/10.1016/j.ecoenv.2020.111654

Muranaka, S., Shono, M., & Takahashi, H. (2016). Nutritional composition and agronomic importance of cowpea (Vigna unguiculata). Legume Research, 39(2), 161–168.

Mutlu, S., & Atici, Ö. (2008). Allelopathic effect of Nepeta meyeri Benth. extracts on seed germination and seedling growth of some crop plants. Acta Physiologiae Plantarum, 31(1), 89–93. https://doi.org/10.1007/s11738-008-0204-0

Oraon, S., & Mondal, S. (2021). Allelopathic effect of lamiaceous weeds on seed germination and early growth of aromatic rice (Oryza sativa ‘Gobindobhog’). Acta Agrobotanica, 74, 741. https://doi.org/10.5586/aa.741

Popola, K. M., Akinwale, R. O., & Adelusi, A. A. (2020). Allelopathic effect of extracts from selected weeds on germination and seedling growth of cowpea (Vigna unguiculata (L.) Walp.) varieties. African Journal of Plant Science, 14(9), 338–349. https://doi.org/10.5897/AJPS2020.2024

Seligler, D. S. (1996). Chemistry and mechanisms of allelopathic interactions. Agronomy Journal, 88(6), 876–885.

Published

2025-12-30

How to Cite

Etuwoma, P. O., & Omakor, I. A. (2025). Growth Response of Two Cowpea Varieties to the Allelopathic Effects of Azadirachta indica (A. Juss) Bark Extract. Faculty of Natural and Applied Sciences Journal of Applied Biological Sciences, 2(4), 60–67. https://doi.org/10.63561/jabs.v2i4.1009